Development of luciferase-linked antibody capture assay based on luciferase immunoprecipitation systems for antibody detection of porcine reproductive and respiratory syndrome virus
نویسندگان
چکیده
منابع مشابه
Antibody profiling by Luciferase Immunoprecipitation Systems (LIPS).
Technologies for comprehensively understanding and quantifying antibody profiles to autoantigens and infectious agents may yield new insights into disease mechanisms and may elucidate new markers to substratify disease with different clinical features and better understand pathogenesis. We have developed a highly quantitative method called Luciferase Immunoprecipitation Systems (LIPS) for profi...
متن کاملluciferase immunoprecipitation system assay, a rapid, simple, quantitative, and highly sensitive antibody detection for parasitic diseases
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متن کاملStandardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...
متن کاملLuciferase Immunoprecipitation System Assay, a Rapid, Simple, Quantitative, and Highly Sensitive Antibody Detection for Parasitic Diseases
tudying immunoglobulin responses by serological techniques is imperative for evaluating antibody titer values during the course of some parasitic infections. Therefore, the presence of a rapid, high-throughput, cost-effective and safe method, which can minimize both false positive-and false negativecases, is beneficial for early diagnosis and accurate treatment of patient. Accordingly, the aim ...
متن کاملPorcine reproductive and respiratory syndrome antibody detection on filter discs.
Two enzyme-linked immunosorbent assay (ELISA) kits were evaluated for detection of porcine reproductive and respiratory syndrome (PRRS) antibodies in pooled sera and filter discs (FDs). Elution and incubation procedures and positive thresholds for both ELISAs were determined using FDs collected from sixty non-infected pigs and five pigs with low PRRS-antibody titres. Eighty paired samples (seru...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2018
ISSN: 1472-6750
DOI: 10.1186/s12896-018-0483-5